Home > Research Team > Josep Villanueva
Current positions
- Head of the Tumor Biomarkers Program
- He also serves in the editorial board of J. of Proteomics.
- Regular ad hoc reviewer for: Journal of Chromatography A; Journal of Proteome Research; Journal of Proteomics; Molecular & Cellular Proteomics; Proteomics; Rapid Communications in Mass Spectrometry.
Academic Qualifications
- Senior Research Scientist with Paul Tempst, 2005-2009. Memorial Sloan-Kettering Cancer Center (MSKCC), New York, USA.
- Postdoctoral Fellow with Paul Tempst, 2002-2005. Memorial Sloan-Kettering Cancer Center (MSKCC), New York, USA.
- Post-doctoral fellow with Luis Serrano, 2002. European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
- Postdoctoral Fellow with Xavier Avilés, 2000-2002. Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.
- Ph.D. in Biochemistry and Molecular Biology with Xavier Avilés and Enrique Querol, 2000. Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.
- Degree in Biochemistry, 1995. Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.
Areas of Research
- Discovery of Secreted Tumor-specific Biomarkers in Breast Cancer by Proteomics.
- Establishment of Secreted Pathway-activation proteomic signatures from signaling pathways linked to tumorigenesis.
- Characterization of the tumor microenvironment by the proteomic profiling of secretomes.
Prizes and Scholarships
- UAB Outstanding Graduate Research Award, Universitat Autònoma de Barcelona, Spain, 2005.
- UAB Graduate Research Fellowship, Universitat Autònoma de Barcelona, Spain, 1996.
- EMBL Short-term Postdoctoral Fellowship, European Molecular Biology Laboratory (EMBL), Germany, 2002.
- U24 CA126485-01 "Clinical Proteomics Technology Assessment for Cancer", NIH National Cancer Institute (2006-2011). Role: Co-Investigator
- Miguel Servet Project. “Quantitative Proteomic Profiling of Secretomes for Drug Target Discovery”. ISCIII (Instituto de la Salud Carlos III) (2009-2011). Role:PI
- Instituto de la Salud Carlos III (FIS).“Pathway-based Secretomes in Breast Cancer Biomarker Discovery” (2010-2012).
- International Reintegration Grant-Marie Curie. “Pathway-based Secretomes in Breast Cancer Biomarker Discovery” (2010-2012).
Most relevant scientific publications
- Villanueva J, Nazarian A, Lawlor K, Tempst P. Monitoring peptidase activities in complex proteomes by MALDI-TOF mass spectrometry. Nat Protoc 2009; 4(8): 1167-83
- Lawlor K, Nazarian A, Lacomis L, Tempst P, Villanueva J. Pathway-based biomarker search by high-throughput proteomics profiling of secretomes. J. Proteome Res. 2009 Mar; 8(3): 1489-503
- Villanueva J, Nazarian A, Lawlor K, Yi SS, Robbins RJ, Tempst P. A sequence-specific exopeptidase activity test (SSEAT) for "functional" biomarker discovery. Mol. Cell Proteomics 2008 Mar; 7(3): 509-18
- Villanueva J, Philip J, DeNoyer L, Tempst P. Data analysis of assorted serum peptidome profiles. Nat Protoc 2007; 2(3): 588-602
- Villanueva J, Lawlor K, Toledo-Crow R, Tempst P. Automated serum peptide profiling. Nat Protoc 2006; 1(2): 880-91
- Villanueva J, Martorella AJ, Lawlor K, Philip J, Fleisher M, Robbins RJ, Tempst P. Serum peptidome patterns that distinguish metastatic thyroid carcinoma from cancer-free controls are unbiased by gender and age. Mol. Cell Proteomics 2006 Oct; 5(10): 1840-52
- Villanueva J, Shaffer DR, Philip J, Chaparro CA, Erdjument-Bromage H, Olshen AB, Fleisher M, Lilja H, Brogi E, Boyd J, Sánchez-Carbayo M, Holland EC, Cordon-Cardo C, Scher HI, Tempst P. Differential exoprotease activities confer tumor-specific serum peptidome patterns. J. Clin. Invest. 2006 Jan; 116(1): 271-84
- Villanueva J, Philip J, Entenberg D, Chaparro CA, Tanwar MK, Holland EC, Tempst P. Serum peptide profiling by magnetic particle-assisted, automated sample processing and MALDI-TOF mass spectrometry. Anal. Chem. 2004 Mar; 76(6): 1560-70
